Intravesical electromotive botulinum toxin administration – new approach of OAB and BPS treatment
Abstract: Hypothesis/aims of study: During more than 15 years, injections of botulinum toxin into detrusor, used for overactive bladder and bladder pain syndrome treatment. Disadvantages of this method: invasiveness, general anaesthesia, patients’ fearing. On this reason, we decided to conduct this study. Electromotive drug administration is widely used method of local drugs administration. Molecule of botulinum toxin A (BOTOX) is too heavy (900 kDa) but incobotulinumtoxinA (Xeomin) molecular weight is only 150 kDa. The aim of our study – evaluate the possibility of electromotive Xeomin administration.
Study design, materials, and methods: We have evaluated mobility molecules of incobotulinumtoxinA in special device for analytical electrophoresis. There were 11 samples with pH: 5.0; 5.4; 5.8; 6.2; 6.6; 7.0; 7.4; 7.8; 8.2; 8.7; 9.0. Distilled water was used as dissolvent with 0.1N HCl or NaOH. Electric power was 5.0 Watt.
Results: Maximal mobility was in sample with pH 5.4, from anode. There was the solution: 100 U incobotulinumtoxinA, 10 ml distilled water with 100 mcl 0.1 N HCl and 1 ml DMSO.
Interpretation of results: Our in vitro experiment has demonstrated possibility of electromotive incobotulinumtoxinA administration. We have developed solution for this procedure: 100 U of Xeomin dissolve in 10 ml distilled water with 100 mcl 0.1 N HCl and 1 ml DMSO.
Concluding message: There is a possibility, that intravesical electromotive incobotulinumtoxinA administration through special catheter-electrode can be used for treatment of OAB and BPS after randomised clinical trials.
Magazine «The Botulinum J.»